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The mRNA vaccines can be widely used in infectious diseases, tumors, and protein replacement therapies:
1) Infectious diseases. The mRNA vaccine can target the conserved region of the virus, directly express specific antigens in cells, activate the body's immune response to produce antibodies, so as to achieve the purpose of preventing infectious diseases;
2) Anti-tumor field. Anti-tumor mRNA vaccines are generally divided into two categories according to the mechanism of action, mRNA vaccine based on dendritic cells (DC) administration and mRNA vaccine for direct injection;
3) Field of protein replacement therapy. To treat some rare diseases by turning the human body into its own protein processing plant.
Plasmid production is an important step in mRNA vaccine production, and the growth rule and metabolic pathway of E. coli are applied to enable the amplification of foreign gene plasmid in E. coli. Using plasmid DNA molecules can be stably in the extrachromosomal free state, replicated with chromosome replication, and transmitted to the offspring by cell division, E. coli is cultured to express the recombinant plasmid in E. coli.CEKG SKC700 and SKC900 bioreactor can be applied to microbial fermentation, microbial fermentation for the plasmid into the corresponding E. coli strains, first through the expanded culture to obtain a large number of E. coli containing plasmids, to help biological pharmaceutical enterprises to adjust production capacity, for the traditional vaccine and application of recombinant technology emerging vaccine production to provide support.
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